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Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue colour
When the sample containing JAM-A is added to the plate
You can calculate the concentration of Rat Cys-C in the samples by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IL31
You can calculate the concentration of Human SPX in the samples by comparing the OD of the samples to the standard curve
Mouse BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit GHRIF Horseradish peroxidase (HRP) bound inThis Mouse BMP 2 (Bone Morphogenetic Protein 2) ELISA Kit uses the sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse BMP 2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse BMP 2 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro
