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The OD value is proportional to the concentration of Human ISG15
Substrate solution is then added and color develops in proportion to the amount of Human GLRX2
a horseradish peroxidase conjugated mouse anti-Human PDGFRA Antibody is added to the wells and binds to PDGFRA in the sample
And then biotinylated Bovine TXNRD1 Antibody is added and binds to TXNRD1 in the sample
You can calculate the concentration of Mouse HMGB-1 in the samples by comparing the OD of the samples to the standard curve
Rat OCN (Osteocalcin) ELISA Kit Packaging:96 wells The OD value is proportionalThe Rat (OCN) Osteocalcin ELISA Kit measures Osteocalcin in Rat samples. The plate has been pre coated with Rat OT antibody. OT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat OT Antibody is added and binds to OT in the sample. Then Streptavidin HRP is added and binds to the Biotinylated OT antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate solution
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