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Luciferase catalyzes the reaction: D-firefly luciferin + O2 + ATP = firefly oxyluciferin + CO2 + AMP + diphosphate + light
The concentration of Rat CTSB in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Human BAX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Small Subunit 1) ELISA Kit is a Sandwich enzyme immunoassay
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CNDP1
Human CALB (Calbindin) ELISA Kit RO Luciferase catalyzes the reaction: D-fireflyThe Human (CALB) Calbindin ELISA Kit measures Calbindin in samples. The plate has been pre coated with Human CALB1 antibody. CALB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CALB1 Antibody is added and binds to CALB1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CALB1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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