100 mL Trypan Blue Solution (w/v) in PBS 0.4% (w/v) in normal saline (8.1 g/L NaCl with 0.6 g/L K2HPO4) 676252 Microscope Primovert with binocular tube

Microscope Primovert with binocular tube for transmitted-light brightfield and phase contrast with objectives 4x

Digital timer with sound alarm 1 min to 96 hours

Cooling Thermo Shaker MKR 23 works on the basis of Smart Control:

Round bottom flask capacities of between 50ml to 5000ml

glass door

100 mL Trypan Blue Solution (w/v) in PBS 0.4% (w/v) in normal saline (8.1 g/L NaCl with 0.6 g/L K2HPO4) 676252 Microscope Primovert with binocular tube0. 4% (w v) in normal saline (8. 1 g L NaCl with 0. 6 g L K2HPO4) Technical Advantage: Can be used for a variety cell counting methods requiring information on cell count and viability. Trypan Blue is the most common stain used in cell count and viability assays. Viable cells exclude the dye, while nonviable cells absorb the dye and appear blue. Cells should be in suspension as single cells in buffered saline before counting. Trypan blue can be used