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Substrate solution is then added and color develops in proportion to the amount of Rat CXCL2
You can calculate the concentration of Rat PTGS2/COX-2 in the samples by comparing the OD of the samples to the standard curve
it binds with VAPA bound to precoated antibody
Then Streptavidin-HRP is added and binds to the Biotinylated PIGR antibody
it binds with ENO3 bound to precoated antibody
Rat MCP-1 (Monocyte Chemotactic Protein 1) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Rat MCP 1 (Monocyte Chemotactic Protein 1) ELISA Kit measures MCP 1 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti MCP 1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
