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When the sample containing Angiopoietin-2 is added to the plate
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit IL18
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle PLGF
RBP3 present in the sample is added and binds to antibodies coated on the wells
then added a HRP-conjugated antibody specific to Human GH
Rat AP (Apelin) ELISA Kit Packaging:96 wells When the sample containing Angiopoietin-2The Rat (AP) Apelin ELISA Kit measures Apelin in Rat samples. The plate has been pre coated with Rat APLN antibody. APLN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat APLN Antibody is added and binds to APLN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated APLN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate solution
