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Then a biotinylated detection antibody specific for Human PKM2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The assay plate has been pre-coated with mouse anti-Mouse CDCP1 monoclonal antibody
MANF present in the sample is added and binds to antibodies coated on the wells
a capture antibody-c-myc-biotin-detection antibody complex was formed
And then biotinylated Human PLA2G2A Antibody is added and binds to PLA2G2A in the sample
Human DTX1 (Protein deltex-1) ELISA Kit SCYB1 Then a biotinylated detection antibodyThe Human DTX1 (Protein deltex 1) ELISA Kit measures DTX1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti DTX1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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