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And then biotinylated Human GMPS Antibody is added and binds to GMPS in the sample
The concentration of Rat HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve
And then biotinylated Mouse GSN Antibody is added and binds to GSN in the sample
And then biotinylated Rat FGFR4 Antibody is added and binds to FGFR4 in the sample
Calculate the concentration of GNAS in the sample by plotting standard curve
Human IAA (Insulin Autoantibodies) ELISA Kit Packaging:96 wells And then biotinylated Human GMPSThe Human IAA (Insulin Autoantibodies) ELISA Kit measures IAA in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP
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