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Then a biotinylated detection antibody specific for Human RBP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
it binds with ORM2 bound to precoated antibody
The plate has been pre-coated with Mouse CCL12 antibody
it binds with NOTCH2 bound to precoated antibody
Then Streptavidin-HRP is added and binds to the Biotinylated IL17C antibody
Rat ADORA2A (Adenosine A2a Receptor) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Rat ADORA2A (Adenosine A2a Receptor) ELISA Kit measures ADORA2A in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ADORA2a antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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