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CCL26 present in the sample is added and binds to antibodies coated on the wells
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle RANTES
Substrate solution is then added and color develops in proportion to the amount of Mouse CLIC1
The concentration of Mouse IgG1 in the samples is then determined by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated PLA2G12A antibody
Human TMPRSS4 (Transmembrane Protease Serine 4) ELISA Kit Packaging:96 wells CCL26 present in the sampleThe Human (TMPRSS4) Transmembrane Protease Serine 4 ELISA Kit measures Transmembrane protease serine 4 in samples. The plate has been pre coated with Human TMPRSS4 antibody. TMPRSS4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TMPRSS4 Antibody is added and binds to TMPRSS4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated TMPRSS4 antibody. After incubation unbound
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