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the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-colored product that is inversely proportional to the amount of total estrogens present
Substrate solution is then added and color develops in proportion to the amount of Human ISLR
Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human E3 are added to the micro ELISA plate wells
Only those wells that contain Human IGFBP-7
Substrate solution is then added and color develops in proportion to the amount of Human LCN12
Rat SLC30A1 (Zinc transporter 1) ELISA Kit Packaging:96 wells the TMB substrate (enzyme substrate)The Rat SLC30A1 (Zinc transporter 1) ELISA Kit measures SLC30A1 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti SLC30A1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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