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The concentration of PGE2 in the samples is then determined by comparing the OD of the samples to the standard curve
coli expression system and the target gene encoding Val77-Leu233 is expressed with a 6His tag at the C-terminus
Then a biotinylated detection antibody specific for Human IFN-Beta and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The OD value is proportional to the concentration of Human BDNF
only those wells that contain Human GH and HRP-conjugated antibody will exhibit a change in color
Human FGF21 (Fibroblast Growth Factor 21) ELISA Kit qPCR (SYBR Green) The concentration of PGE2 inThis Fibroblast Growth Factor 21 ELISA kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human FGF21. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF21 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and
