Human E2 (Estradiol) ELISA Kit Packaging:96 wells The concentration of Mouse ES

The concentration of Mouse ES in the samples is then determined by comparing the OD of the samples to the standard curve

Then a biotinylated detection antibody specific for Rat SDF-1/CXCL12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Then a biotinylated detection antibody specific for Human F7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

and the color intensity is directly proportional to the amount of Human IL-29 present

Then a biotinylated detection antibody specific for Human sVEGFR-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Human E2 (Estradiol) ELISA Kit Packaging:96 wells The concentration of Mouse ESThe Human (E2) Estradiol ELISA Kit measures Estradiol in samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added and color develops. The