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The concentration of Human GPR35 in the samples is then determined by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Rabbit EGFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
it binds with IGFBP3 bound to precoated antibody
If the sample contains Gls
Then Streptavidin-HRP is added and binds to the Biotinylated CEACAM4 antibody
Rat PCIII (Procollagen III) ELISA Kit 42026 The concentration of Human GPR35The Rat PCIII (Procollagen III) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat PCIII. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PCIII and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well
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