Rat PROG (Progesterone) ELISA Kit Packaging:96 wells Recombinant Human Insulin-Like Growth Factor-Binding

Recombinant Human Insulin-Like Growth Factor-Binding Protein 5 is produced by our Mammalian expression system and the target gene encoding Leu21-Glu272 is expressed with a 6His tag at the C-terminus

And then biotinylated Rat HYAL1 Antibody is added and binds to HYAL1 in the sample

Then a biotinylated detection antibody specific for Mouse S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

FASN present in the sample is added and binds to antibodies coated on the wells

Then Streptavidin-HRP is added and binds to the Biotinylated MUSK antibody

Rat PROG (Progesterone) ELISA Kit Packaging:96 wells Recombinant Human Insulin-Like Growth Factor-BindingThe Rat (PROG) Progesterone ELISA Kit measures Progesterone in Rat samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added and color