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Then a biotinylated detection antibody specific for Rat IgG2c and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
And then biotinylated Mouse FETUB Antibody is added and binds to FETUB in the sample
Calculate the concentration of SCN9A/Nav1
Then Streptavidin-HRP is added and binds to the Biotinylated FLOT1 antibody
Calculate the concentration of VAPA in the sample by plotting standard curve
Human TTRAP (TRAF and TNF receptor-associated protein) ELISA Kit 4-1BB Then a biotinylated detection antibodyThe Human TTRAP (TRAF and TNF receptor associated protein) ELISA Kit measures TTRAP in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TTRAP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add
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