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The assay plate has been pre-coated with mouse anti-Human CD200 monoclonal antibody
You can calculate the concentration of ANG2 in the samples by comparing the OD of the samples to the standard curve
CSPG4 present in the sample is added and binds to antibodies coated on the wells
The plate has been pre-coated with Rat TIMP2 antibody
Then Streptavidin-HRP is added and binds to the Biotinylated GLB1 antibody
Rat WNT5A (Protein Wnt-5A) ELISA Kit Reagents The assay plate has beenThe Rat (WNT5A) Protein Wnt 5A ELISA Kit measures Protein Wnt 5a in Rat samples. The plate has been pre coated with Rat WNT5A antibody. WNT5A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat WNT5A Antibody is added and binds to WNT5A in the sample. Then Streptavidin HRP is added and binds to the Biotinylated WNT5A antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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