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Then a biotinylated detection antibody specific for Rat CKMB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human GAL2 in the samples is then determined by comparing the OD of the samples to the standard curve
KIRREL present in the sample is added and binds to antibodies coated on the wells
This LTF (Lactoferrin) ELISA Kit uses the sandwich-ELISA principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse C3a
Human PLD2 (Phospholipase D2) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human (PLD2) Phospholipase D2 ELISA Kit measures Phospholipase D2 in samples. The plate has been pre coated with Human PLD2 antibody. PLD2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PLD2 Antibody is added and binds to PLD2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PLD2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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