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Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgG are added to the micro ELISA plate wells
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IFN-Beta
The concentration of Universal 5-HIAA in tested samples can be calculated by comparing the OD of the samples to the standard curve
The OD value is proportional to the concentration of Human MAU
Then a biotinylated detection antibody specific for Human SEPP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human IFN-alpha10 ELISA Kit Packaging:48 wells Samples (or Standards) and HorseradishThe Human IFN alpha10 ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human IFNa10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human IFNa10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,
