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The Rat MMP13 (Matrix Metalloproteinase 13) ELISA Kit is a Sandwich enzyme immunoassay
Mg2+ and other components
coli expression system and the target gene encoding His23-Ser140 is expressed
The concentration of Rat IL20 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GAN
Rat PGRN (Progranulin) ELISA Kit NAP1 The Rat MMP13 (Matrix MetalloproteinaseThe Rat (PGRN) Progranulin ELISA Kit measures Progranulin in Rat samples. The plate has been pre coated with Rat GRN antibody. GRN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat GRN Antibody is added and binds to GRN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GRN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
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