Active Caspase 3 Mouse mAb Flt3-L Only those wells that contain

Only those wells that contain Human TTR

You can calculate the concentration of Rat NSE in the samples by comparing the OD of the samples to the standard curve

Human IgG1 in the sample or standard competes with a fixed amount of Human IgG1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human IgG1

and the Rat PG standard plate wells that pre-coated using protein-related techniques are provided separately

Then a biotinylated detection antibody specific for Rat IgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Active Caspase 3 Mouse mAb Flt3-L Only those wells that containActivation of caspase 3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase 3 requires aspartic acid at the P1 position.