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You can calculate the concentration of Human CTLA4 in the samples by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated IL-5 antibody
And then biotinylated Rat C1QB Antibody is added and binds to C1QB in the sample
CSH2 present in the sample is added and binds to antibodies coated on the wells
BTC/betacellulin
Human RFTN2 (Raftlin-2) ELISA Kit Packaging:96 wells You can calculate the concentrationThe Human (RFTN2) Raftlin 2 ELISA Kit measures Raftlin 2 in samples. The plate has been pre coated with Human RFTN2 antibody. RFTN2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human RFTN2 Antibody is added and binds to RFTN2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated RFTN2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
