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Then Streptavidin-HRP is added and binds to the Biotinylated SERPINF1 antibody
it binds with DEK bound to precoated antibody
HRC present in the sample is added and binds to antibodies coated on the wells
Calculate the concentration of TGF-β2 in the sample by plotting standard curve
The OD value is proportional to the concentration of Rat IGFBP-5
Human KNG1 (Kininogen 1) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Human KNG1 (Kininogen 1) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human KNG1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human KNG1 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well
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