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The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LTF
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD97
The concentration of Human ACTN1 in the samples is then determined by comparing the OD of the samples to the standard curve
And then biotinylated Human ADAMTS19 Antibody is added and binds to ADAMTS19 in the sample
The MYL1 (Myosin Light Chain 1
Rat CLDN5 (Claudin-5) ELISA Kit ADAM metallopeptidase domain 17 The micro ELISA plate providedThe Rat (CLDN5) Claudin 5 ELISA Kit measures Claudin 5 in Rat samples. The plate has been pre coated with Rat CLDN5 antibody. CLDN5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat CLDN5 Antibody is added and binds to CLDN5 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CLDN5 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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