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it binds with DEFA3 bound to precoated antibody
then it binds with OVM-IgA bound to precoated antibody
Then Streptavidin-HRP is added and binds to the Biotinylated TGFA antibody
And then biotinylated Mouse SELE Antibody is added and binds to SELE in the sample
Substrate solution is then added and color develops in proportion to the amount of Human KLF15
Human OCLN (Occludin) ELISA Kit KSP3 it binds with DEFA3 boundThe Human OCLN (Occludin) ELISA Kit measures OCLN in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti OCLN antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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