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LMX1B in the sample or standard competes with a fixed amount of biotin-labeled LMX1B for sites on a pre-coated antibody specific to LMX1B
it binds with EIF6 bound to precoated antibody
Then Streptavidin-HRP is added and binds to the Biotinylated CNTF antibody
The microplate provided in this kit has been precoated with anti CDK4 antibody
The OD value is proportional to the concentration of Mouse CTXI
Human SEPT5 (Septin-5) ELISA Kit Packaging:96 wells LMX1B in the sample orThe Human SEPT5 (Septin 5) ELISA Kit measures SEPT5 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti SEPT5 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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