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The OD value is proportional to the concentration of Human IL-16
then added a HRP-conjugated antibody specific to Human ANXA1
Then a biotinylated detection antibody specific for Mouse VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human Irisin in the samples is then determined by comparing the OD of the samples to the standard curve
The concentration of Cattle IL5 in the samples is then determined by comparing the OD of the samples to the standard curve
Human LTb (Lymphotoxin Beta) ELISA Kit SRIF The OD value is proportionalThe Human LTb (Lymphotoxin Beta) ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human LTb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human LTb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is
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