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Then a biotinylated detection antibody specific for Human IL-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated Lptn antibody
And then biotinylated Human SELL Antibody is added and binds to SELL in the sample
NPHS1 present in the sample is added and binds to antibodies coated on the wells
The plate has been pre-coated with Rat NR3C1 antibody
Human CNTF (Ciliary neurotrophic factor) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human CNTF (Ciliary neurotrophic factor) ELISA Kit measures CNTF in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CNTF antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
