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The concentration of Rabbit IL8 in the samples is then determined by comparing the OD of the samples to the standard curve
GCLM present in the sample is added and binds to antibodies coated on the wells
The human EDTA plasma is incubated in the plate with the capture antibody
Then Streptavidin-HRP is added and binds to the Biotinylated CS antibody
The OD value is proportional to the concentration of MMP-9
Human PLS1 (Plastin-1) ELISA Kit CKM The concentration of Rabbit IL8The Human (PLS1) Plastin 1 ELISA Kit measures Plastin 1 in samples. The plate has been pre coated with Human PLS1 antibody. PLS1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PLS1 Antibody is added and binds to PLS1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PLS1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
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