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Then a biotinylated detection antibody specific for Human Cyt-C and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PP
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ALCAM
The concentration of Human OPTN in the samples is then determined by comparing the OD of the samples to the standard curve
The EMP3 (Epithelial Membrane Protein 3) ELISA Kit is a Sandwich enzyme immunoassay
Human CGN (Cingulin) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human (CGN) Cingulin ELISA Kit measures Cingulin in samples. The plate has been pre coated with Human CGN antibody. CGN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CGN Antibody is added and binds to CGN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CGN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate solution
